Schedule: Environmental Impacts on Genome Stability

 
Tuesday, June 19th
9:00 -   9:30:   Workshop overview.

9:30 - 10:15:  Plant genome and its body. (Marc Curtis)

1.      Life-cycle

2.      Life-cycle: plants vs. animals

3.      The Genome

                                    4.   Genotype to phenotype

5.      Genome maintenance/stability functions

6.      Advantages of Arabidopsis as system of study

 
10:15-10:30: Questions/Discussion.
 
10:30-11:50:  In the lab:

                                    Observing aspects of the plant life-cycle: microscopy and

                                    time-lapse photography

 

                                    Toxin infiltration of Arabidopsis leaves

 

11:50 - 1:30: Lunch

 
1:30 - 2:15: Arabidopsis and seed biology. (Jessica Hewitt)
 
2:15 - 2:30: Questions/Discussion.
 

2:30 - 4:00:  In the lab.

1.      Preparing agar plates

2.      Sowing seed on soil or agar plates

3.      Growth conditions

4.      Begin processing leaves for GUS spots   

 
 
 
 
 
 
 
 
 
 
Wednesday, June 20th

9:00 - 9:45:    A perilous world for the genome. (Marc Curtis)

1.      DNA damage

a.       Endogenous

b.      Exogenous

                                                                                                                           i.      Abiotic stress

                                                                                                                         ii.      Biotic stress

 
9:45 - 10:00: Questions/Discussion.  
 
 

10:00-10:45: Arabidopsis and plant disease – biotic stress. (Jenny Lorang)

 
10:45-11:00: Questions/Discussion.
 
11:00-11:50: In the lab:

                                    Ultraviolet irradiation (abiotic stress) of plants

                                    Observe previously irradiated plants 

 

11:50 - 1:30: Lunch

 
1:30 - 2:15: Maintaining genome integrity. (John Hays)

1.      DNA repair

2.      Mutagenic pathways

 
2:15 - 2:30: Questions/Discussion
 
2:30 - 4:00:   In the lab

Web resources: How to browse a genome database

TAIR website  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Thursday, June 21st

9:00 - 9:20:   Genotype to phenotype: Mutants and natural variation (Marc Curtis)

a.       classic breeding

b.      induced-mutagenesis

c.       transgenics

 

9:20 - 9:45:   Transgenics in basic research and agriculture (Walt Ream)

 
9:45 -10:00:   Questions/Discussion
 

10:00-10:25: Arabidopsis and genomics (Elizabeth Chapman)

                                    Sequencing, microarrays and RNAs

                                   
11:25-11:00: Tour CGRB
 

11:00-11:35: Arabidopsis natural variation and resistance gene evolution

(Erica Bakker)
 
11:35-11:50: Questions/Discussion
 
11:50 - 1:30:   Lunch
 
1:30 - 3:00:   In the lab

1)      Finish processing leaves for GUS spots

2)      Scoring GUS spots

 

3:00 - 4:00:   Critique, feedback, suggestions, discussion, plan of action.