Forensics/PCR Workshop

PCR-based Crime Scene Investigations in the High School Classroom

Date:  June 26-28, 2006  

Location:  Oregon State University, ALS 012

Instructors:  Drs. Kirstin Carroll and Kari van Zee

Course Description:

The intent of this curriculum is to guide students through
the thought process involved in a laboratory-based scientific investigation.
Students will learn about PCR, gel electrophoresis, genotyping, and genotype
matching while asking the question "How can a tiny amount of genetic material
(DNA) be used to identify one person out of a billion?"
We will pilot the Bio-Rad Explorer Crime Scene Investigator
PCR Basics™ Kit in this activity.  The kit is appropriate for students who have
little or no experience with molecular biology or PCR, but may also be suitable
for more advanced students with an interest in the details of DNA profiling,
forensic science, and statistics. Students who have an understanding of the
following concepts would fully appreciate the kit:
• The structure of DNA
• Genotypes and genotyping
• Heredity and the passage of genetic information from parents to offspring
• DNA replication and PCR
• Cell structure and the storage of DNA within the nucleus
• Pattern matching and discrimination